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The School of Mining and Agriculture, established in 1893, was designed to provide Queen's with an engineering faculty. The provincial government was not permitted to provide Queen's with any funding as long as it was a denominational university, which it remained until 1912. But Principal George Munro Grant, and Premier Oliver Mowat (son of one of Queen's founders and brother of one of its senior professors), got around this technicality by establishing the provincially-supported School as an independent institution. Sharing the newly-built Carruthers Hall, as well as its professors with Queen's Faculty of Applied Science, that latter was deliberately founded at the same time.

The School of Mining and Agriculture opened in 1893, and the faculty was created in 1894, with Nathan Dupuis as its first Dean. There was some dispute about what to name the new faculty, and, although it was officially called the Faculty of Applied Science, it was usually referred to as the Faculty of Practical Science (then the most common term elsewhere) in its early years. The School of Mining vanished from the campus scene in 1916, when it and the faculty united to become the Faculty of Applied Science. In 1993, the Faculty celebrated its Centennial Anniversary.

After Arts and Science, the Faculty of Applied Science is the second largest on campus, with close to 4600 students at the undergraduate level. Its students have long been known for their boisterous faculty spirit and are easily identified on campus by their dyed purple leather jackets.

In March 2010, and upon the formal approval of the University Senate, the name of the Faculty of Applied Science was changed to the Faculty of Engineering and Applied Science. In the words of the Dean, Kimberley Woodhouse, "by including [the word] 'Engineering' in our name, we more accruately reflect what we do and who we are."

The Faculty of Health Sciences was established in 1854, after more than a decade of effort by Queen's officials to add a medical school to the young University. It began in a small limestone house at 75 Princess Street, soon thereafter moving to Summerhill, where the rest of the University was located. In 1858, it moved into the first permanent building that Queen's built for itself: the Old Medical Building. In 1866, however, the Faculty split from the University after medical professors – less theologically-minded than their colleagues – protested against having to make a public declaration of the Presbyterian faith. The Faculty became the Royal College of Physicians and Surgeons, Kingston (RCPSK), which retained a loose affiliation with the University. The RCPSK eventually reunited with Queen's in 1892, in order to share resources and expertise.

The Faculty grew enormously in the 20th century, evolving into one of Canada's premier centres for medical research, as well as teaching. In recent decades, the most important development in medical education was the establishment in the 1960s of the Kingston Health Sciences Centre, which brought the Faculty of Medicine and the School of Nursing together with local hospitals to provide cooperative facilities for exemplary patient care, research, and training. Planning for a nursing program at Queen's began in 1941 The first students were admitted in the Fall of 1942 and the first Director of the School of Nursing was appointed in 1946. In 1979, the School of Rehabilitation Therapy, originally a stand alone unit, became part of the Faculty.

In 1998, the School of Medicine and School of Rehabilitation Therapy were joined by the School of Nursing to become the current Faculty of Health Sciences. Today the Faculty has about 330 full-time faculty members but many more part-time, since every doctor with attending privileges at Kingston General Hospital, Hotel Dieu Hospital, and Providence Continuing Care Centre's St. Mary's of the Lake Hospital site normally holds a faculty appointment in the School of Medicine as well. The Faculty of Health Sciences forms the academic core of the Academic Health Sciences Centre, and is also part of the Health Care Network of Southeastern Ontario. Academic programs are based on campus but are distributed throughout southeastern Ontario's health care facilities, including affiliations with Quinte Healthcare Corporation, Lakeridge Hospital, Peterborough, Perth, Brockville, Weeneebayko (Moose Factory) being amongst many other sites. The innovative Alternative Funding Plan (AFP), a contractual agreement of SEAMO and the Ministry of Health & Long-Term Care and the Ministry of Community & Social Services, provides stable funding for the delivery of research, education and extensive tertiary, secondary and some primary care in a region of over one million people.

The Faculty offers programs in undergraduate and postgraduate medical education; undergraduate education in Physical and Occupational Therapy, and graduate education in Rehabilitation Science; undergraduate and postgraduate education in Nursing, including the Nurse Practitioner Program; graduate education in the Life Sciences; and collaborative programs in Respiratory Therapy and in X-Ray Technology. Main offices of the Faculty are located in Botterell Hall.

Degrees conferred by the Faculty include: Doctor of Medicine (MD), Bachelor of Nursing Sciences (BNSc), Master of Science, Nursing (MSc), Bachelor of Science Physical Therapy (B.Sc. P.T.), Bachelor of Science, Occupational Therapy (B.Sc.O.T.), Master of Science, Rehabilitation Science (M.Sc.), Doctor of Philosophy, Rehabilitation Science (Ph.D.), Master of Science, Life Sciences (M.Sc.), Doctor of Philosophy, Life Sciences (Ph.D.) .Schools under the auspisces of the Faculty of Health Sciences include: Medicine, Nursing, Rehabilitation TherapyDepartments: Anatomy & Cell Biology, Anesthesiology, Biochemistry, Community Health & Epidemiology, Diagnostic Radiology, Emergency Medicine, Family Medicine, Medicine, Microbiology & Immunology, Obstetrics & Gynaecology, Oncology, Opthalmology, Otolaryngology, Pathology, Pediatrics, Pharmcology & Toxicology, Physiology, Psychiatry, Rehabilition Medicine, Surgery and Urology.

The Faculty of Health Sciences established the Tony Travill Debate in memory of Professor A.A.Travill (1925-1996), MBBS(London), MRCS(Eng), LRCP(London), MSc(Med)(Queen's), former head of the Anatomy Department at Queen's University (1969-1978). Dr Travill was an excellent teacher, physician, philosopher and historian, who was a devotee of logical argument and witty debate. This annual event allows medical researchers to debate a controversial topic in medicine from two different perspectives, both supported by research.

This is one of the oldest, as well as one of the newest faculties at Queen's. It was first founded in 1860, but closed down for financial reasons in 1864. It was revived for another three years in the 1880s, but did not become a permanent fixture at the University until 1957, when the Law Society of Upper Canada decentralized the teaching of law in Ontario, allowing lawyers to be taught in institutions other than Osgoode Hall in Toronto. Queen's Principal, William Mackintosh, was a leader of the campaign to convince the Law Society to change its rules, arguing that universities could offer a more varied and wider understanding of law. Vice-Principal (later Principal) James Corry was the first Acting Dean, followed by William Lederman, the first permanent Dean. Corry took on some teaching duties, with Professors Daniel Soberman (LLD'08) and Stewart Ryan (LLD'91) as the faculty's only full-time academic staff for the first class of 24 students. In 2008, the faculty changed its LLB (Bachelor of Laws) designation to a JD (Juris Doctor) designation to align itself with international universities. It also offers a Masters of Law (LL.M) and PhD programs through the School of Graduate Studies.

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The Gaels Hoop Club was a fundraising organization established in 1982 to support the Queen's University varsity basketball team.

Officially known as the George L. Edgett Statistical Laboratory, (STATLAB) was a statistical consulting facility available to all members of the Queen's community --researchers, graduate and undergraduate students, and staff, as well as to clients outside of the University. STATLAB was established the Queen's Senate in 1973, and was one of the first such units in Canada. In 1979, the facility was named after Professor George L. Edgett, a former member of the Department of Mathematics and Statistics, and one of the country's first university statisticians. The Lab was maintained by a Director, appointed by the Dean of Arts and Science. Statisticians in the Department of Mathematics and Statistics served as the senior consultant, one per term; while graduate students in statistics staffed a drop-in centre. Consulting was offered on most problems involving statitiscal issues, including the formulation of research questions in quantitative terms; the verificaiton of reports incorporating results from computer output, and the creation of new statistical procedures. Work on projects was supported by grants, contracts, or administrative funds, and was chargeable. All external clients paid consulting fees. The George E. Edgett Statistical Laboratory, which was located on the University campus in Jeffrey Hall, suspended operations in 1998.

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