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QUFA is the exclusive bargaining agent for all faculty, librarians, and archivists at Queen's University and Queen's Theological College. From its founding in 1951 until 1995 QUFA was a non-unionized Faculty Association organized by the professorate to protect collective and individual rights, with librarians and archivists joining in 1977. In November 1995, the majority of faculty as well as professional librarians and archivists at Queen's were organized by QUFA into a collective bargaining unit under the Ontario Labour Relations Board. In May 2003 QUFA added a second bargaining unit, called Sessional Adjuncts, who teach either individually or jointly for pay. With only a very few exceptions, all Queen's University faculty were automatically members of one of the two bargaining units. In July 2007, the two bargaining units amalgamated to form one bargaining unit. The definition of individuals who are included in the bargaining unit is available on QUFA's website in Article 1 of the Collective Agreement 2008-2011. In April 2009 QUFA became a multi-employer union when it added a bargaining unit comprising academic staff at Queen's School of Religion (QSR). In the spring of 2012, QSR became part of Queen's University and those members joined the main unit to form a single bargaining unit of Faculty, Librarians and Archivists (FLA).

This Faculty traces its origins to Queen's Royal Charter of 1841, which declared that the University would both train students as Presbyterian ministers and instruct youth "in the various branches in Science and Literature." In the University's first 30 or 40 years, however, there was no hard and fast distinction between arts and theology, and professors taught in both faculties.
Queen's first two professors, the Rev Thomas Liddell and the Rev Peter Colin Campbell, initiated the Arts and Science curriculum with courses in three subjects: Classics, Mathematics, and Natural Philosophy (the Victorian term for Natural Science). By the end of the 19th century, the faculty also offered courses in Chemistry, English, French, German, History, Philosophy, Physics, Political and Economic Science (since divided into Economics and Political Studies), and Psychology.
Today, the Faculty offers a broad range of undergraduate degree programs in the sciences, social sciences, humanities, fine arts, and languages.

Queen's first Faculty of Education was founded in 1907, but closed in 1920, when the training of teachers in Ontario was centralized in Toronto. The present Faculty dates from 1965, when the province approved the Duncan McArthur College of Education, a Queen's-affiliated college. Named after a former head of the University's Department of History, who became Ontario's Minister of Education, the College registered its first 40 students in the 1968-1969 academic year, under the Deanship of Queen's alumnus Vernon Ready. By 1971, the college had been renamed the Faculty of Education in order to clarify its relationship with Queen's, and had moved to its present home in Duncan McArthur Hall, located at the University's West Campus. The Faculty trains students as teachers in all school subjects and for all levels from Kindergarten to high school. There is also a Concurrent Education (ConEd) program, which combines teacher training with regular undergraduate studies in the Faculty of Arts and Science, or at Trent University in Peterborough, Ontario. The Faculty also offers several alternative education programs: Aboriginal Teacher Education; Artist in Community Education; Continuing Teacher Education; the Cooperative Program in Outdoor and Experiential Education. The Faculty also operates the Queen's School of English, who offers non-credit courses in English as a second language.

The School of Mining and Agriculture, established in 1893, was designed to provide Queen's with an engineering faculty. The provincial government was not permitted to provide Queen's with any funding as long as it was a denominational university, which it remained until 1912. But Principal George Munro Grant, and Premier Oliver Mowat (son of one of Queen's founders and brother of one of its senior professors), got around this technicality by establishing the provincially-supported School as an independent institution. Sharing the newly-built Carruthers Hall, as well as its professors with Queen's Faculty of Applied Science, that latter was deliberately founded at the same time.

The School of Mining and Agriculture opened in 1893, and the faculty was created in 1894, with Nathan Dupuis as its first Dean. There was some dispute about what to name the new faculty, and, although it was officially called the Faculty of Applied Science, it was usually referred to as the Faculty of Practical Science (then the most common term elsewhere) in its early years. The School of Mining vanished from the campus scene in 1916, when it and the faculty united to become the Faculty of Applied Science. In 1993, the Faculty celebrated its Centennial Anniversary.

After Arts and Science, the Faculty of Applied Science is the second largest on campus, with close to 4600 students at the undergraduate level. Its students have long been known for their boisterous faculty spirit and are easily identified on campus by their dyed purple leather jackets.

In March 2010, and upon the formal approval of the University Senate, the name of the Faculty of Applied Science was changed to the Faculty of Engineering and Applied Science. In the words of the Dean, Kimberley Woodhouse, "by including [the word] 'Engineering' in our name, we more accruately reflect what we do and who we are."

The Faculty of Health Sciences was established in 1854, after more than a decade of effort by Queen's officials to add a medical school to the young University. It began in a small limestone house at 75 Princess Street, soon thereafter moving to Summerhill, where the rest of the University was located. In 1858, it moved into the first permanent building that Queen's built for itself: the Old Medical Building. In 1866, however, the Faculty split from the University after medical professors – less theologically-minded than their colleagues – protested against having to make a public declaration of the Presbyterian faith. The Faculty became the Royal College of Physicians and Surgeons, Kingston (RCPSK), which retained a loose affiliation with the University. The RCPSK eventually reunited with Queen's in 1892, in order to share resources and expertise.

The Faculty grew enormously in the 20th century, evolving into one of Canada's premier centres for medical research, as well as teaching. In recent decades, the most important development in medical education was the establishment in the 1960s of the Kingston Health Sciences Centre, which brought the Faculty of Medicine and the School of Nursing together with local hospitals to provide cooperative facilities for exemplary patient care, research, and training. Planning for a nursing program at Queen's began in 1941 The first students were admitted in the Fall of 1942 and the first Director of the School of Nursing was appointed in 1946. In 1979, the School of Rehabilitation Therapy, originally a stand alone unit, became part of the Faculty.

In 1998, the School of Medicine and School of Rehabilitation Therapy were joined by the School of Nursing to become the current Faculty of Health Sciences. Today the Faculty has about 330 full-time faculty members but many more part-time, since every doctor with attending privileges at Kingston General Hospital, Hotel Dieu Hospital, and Providence Continuing Care Centre's St. Mary's of the Lake Hospital site normally holds a faculty appointment in the School of Medicine as well. The Faculty of Health Sciences forms the academic core of the Academic Health Sciences Centre, and is also part of the Health Care Network of Southeastern Ontario. Academic programs are based on campus but are distributed throughout southeastern Ontario's health care facilities, including affiliations with Quinte Healthcare Corporation, Lakeridge Hospital, Peterborough, Perth, Brockville, Weeneebayko (Moose Factory) being amongst many other sites. The innovative Alternative Funding Plan (AFP), a contractual agreement of SEAMO and the Ministry of Health & Long-Term Care and the Ministry of Community & Social Services, provides stable funding for the delivery of research, education and extensive tertiary, secondary and some primary care in a region of over one million people.

The Faculty offers programs in undergraduate and postgraduate medical education; undergraduate education in Physical and Occupational Therapy, and graduate education in Rehabilitation Science; undergraduate and postgraduate education in Nursing, including the Nurse Practitioner Program; graduate education in the Life Sciences; and collaborative programs in Respiratory Therapy and in X-Ray Technology. Main offices of the Faculty are located in Botterell Hall.

Degrees conferred by the Faculty include: Doctor of Medicine (MD), Bachelor of Nursing Sciences (BNSc), Master of Science, Nursing (MSc), Bachelor of Science Physical Therapy (B.Sc. P.T.), Bachelor of Science, Occupational Therapy (B.Sc.O.T.), Master of Science, Rehabilitation Science (M.Sc.), Doctor of Philosophy, Rehabilitation Science (Ph.D.), Master of Science, Life Sciences (M.Sc.), Doctor of Philosophy, Life Sciences (Ph.D.) .Schools under the auspisces of the Faculty of Health Sciences include: Medicine, Nursing, Rehabilitation TherapyDepartments: Anatomy & Cell Biology, Anesthesiology, Biochemistry, Community Health & Epidemiology, Diagnostic Radiology, Emergency Medicine, Family Medicine, Medicine, Microbiology & Immunology, Obstetrics & Gynaecology, Oncology, Opthalmology, Otolaryngology, Pathology, Pediatrics, Pharmcology & Toxicology, Physiology, Psychiatry, Rehabilition Medicine, Surgery and Urology.

The Faculty of Health Sciences established the Tony Travill Debate in memory of Professor A.A.Travill (1925-1996), MBBS(London), MRCS(Eng), LRCP(London), MSc(Med)(Queen's), former head of the Anatomy Department at Queen's University (1969-1978). Dr Travill was an excellent teacher, physician, philosopher and historian, who was a devotee of logical argument and witty debate. This annual event allows medical researchers to debate a controversial topic in medicine from two different perspectives, both supported by research.

This is one of the oldest, as well as one of the newest faculties at Queen's. It was first founded in 1860, but closed down for financial reasons in 1864. It was revived for another three years in the 1880s, but did not become a permanent fixture at the University until 1957, when the Law Society of Upper Canada decentralized the teaching of law in Ontario, allowing lawyers to be taught in institutions other than Osgoode Hall in Toronto. Queen's Principal, William Mackintosh, was a leader of the campaign to convince the Law Society to change its rules, arguing that universities could offer a more varied and wider understanding of law. Vice-Principal (later Principal) James Corry was the first Acting Dean, followed by William Lederman, the first permanent Dean. Corry took on some teaching duties, with Professors Daniel Soberman (LLD'08) and Stewart Ryan (LLD'91) as the faculty's only full-time academic staff for the first class of 24 students. In 2008, the faculty changed its LLB (Bachelor of Laws) designation to a JD (Juris Doctor) designation to align itself with international universities. It also offers a Masters of Law (LL.M) and PhD programs through the School of Graduate Studies.

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The Gaels Hoop Club was a fundraising organization established in 1982 to support the Queen's University varsity basketball team.

Officially known as the George L. Edgett Statistical Laboratory, (STATLAB) was a statistical consulting facility available to all members of the Queen's community --researchers, graduate and undergraduate students, and staff, as well as to clients outside of the University. STATLAB was established the Queen's Senate in 1973, and was one of the first such units in Canada. In 1979, the facility was named after Professor George L. Edgett, a former member of the Department of Mathematics and Statistics, and one of the country's first university statisticians. The Lab was maintained by a Director, appointed by the Dean of Arts and Science. Statisticians in the Department of Mathematics and Statistics served as the senior consultant, one per term; while graduate students in statistics staffed a drop-in centre. Consulting was offered on most problems involving statitiscal issues, including the formulation of research questions in quantitative terms; the verificaiton of reports incorporating results from computer output, and the creation of new statistical procedures. Work on projects was supported by grants, contracts, or administrative funds, and was chargeable. All external clients paid consulting fees. The George E. Edgett Statistical Laboratory, which was located on the University campus in Jeffrey Hall, suspended operations in 1998.